The cell death phenomenon, an abstract
The cell death phenomenon , apart from being important feature in the development of the nervous system .Many neurological diseases are characterized by the gradual loss of specific sets of neurons and result in disorders of movements and CNS functions , such diseases are hypoxic ischemic encephalography . The mechanism of apoptotic neurodegenration in thalamus and other brain regions after neonatal hypoxic ischemia are completely unknown however ,death receptor -activated pathways ,altered mitochondrial function and changes in expression of mitochondrial -related bcl- 2 family proteins are likely important effectors of programmed cell death . Fas /Apo-1(CD95) is an important one of of mitochondrial -related bcl- 2 family proteins. Fas death receptor (CD95) expression increased in hippocampus bilaterally during 24 hours immediately after neonatal hypoxic ischemic encephalopathy . This study was designed to evaluate the apoptotic process in CNS tissue which occurred in neonatal hypoxic ischemic encephalopathy by measuring the levels of Fas in serum and CSF in neonatal hypoxic ischemic encephalopathy . Material and Methods : The study was done on 80 neonates , 60 neonates with hypoxic ischemic encephalopathy were admitted in premature and IC units in AL-minya university hospital and 20 healthy neonates as control group. All neonates were subjected to careful history of HIE .clinically delaminated with assessment of severity of HIE by using Apgar and Thompson score . Laboratory investigation were done to all neonates included :complete blood picture ,C-reactive protein , urine analyses and culture ,CSF by lumber puncture .Fas/Apo-1(CD95) was measured in serum and CSF by a solid-phase sandwich ,two-site, enzyme-linked immunoassay (ELISA). Results: Serum soluble Fas levels in HIE neonates were significantly elevated (15.81± 7.13 ng/dl) in comparing with control group (4.09± 1.15 ng/dl) (p < 0.0001) . Significantly increased in soluble Fas levels in CSF of HIE neonates (3.93± 3.01 ng/dl) when compared with healthy control neonates (0.33± 0.27 ng/dl) ,(p <0.0001). Significant positive role of the prematurity on Fas in serum and CSF ,where a statistically significantly increased in serum and CSF Fas levels in HIE premature neonates (210.9± 5.59 ng/dl) ( 6.62± 2.73 ng/dl) respectively , compared to those of HIE full -term neonates (12.76± 5.76 ng/dl) ( 2.58± 2.11 ng/dl) (p <0.0001). there was a significant correlation between the levels of Fas in serum and CSF with the severity of HIE according to Thompson score. Conclusion: we concluded that an increase in Fas in serum and CSF of HIE in neonates explain the apoptotic process in brain tissue damage in HIE. Also by measuring Fas levels in serum and CSF of HIE neonates we can used it as marker of apoptosis and can detect the severity of HIE.
Authors: Samir Tamer Abdullah*Sawsn Mahmoud Elbana *Tahea Hashem Selem** Aber Mohamed Hassen Noman*
Neurology